ICSI and IMSI for fertilisation
In Vitro Fertilisation of the eggs can be done through conventional IVF or sperm microinjection (ICSI).
What is the conventional fertilisation?
The conventional fertilisation consists of bringing into contact the eggs with more than 100000 spermatozoa and wait for them to be fertilised.
The conventional fertilisation has been the common technique for In Vitro Fertilisation (IVF) until ICSI arrived. Currently its use is limited to those cases in which fertilisation is carried out with both techniques if an elevated number of eggs was obtained.
What is ICSI?
ICSI consists of an Intra Cytoplasmic Sperm Injection (ICSI). One spermatozoon is introduced into each egg through microinjection. The selection of the spermatozoon is done under a microscope with 400 times amplification.
ICSI is the most frequent technique for In Vitro Fertilisation for the majority of cases, especially if there have been previous fertilisation failures or a history of very bad sperm quality.
What is IMSI?
IMSI is the Intra Cytoplasmic Injection of morphologically selected spermatozoon. Other than with ICSI, the selection is done under 8000 times of amplification, which allows choosing only the best sperm. With such amplification, structures can be observed which under a conventional microscope would never be detectable. For this technique, a high quality microscope with perfect image quality and complex IT software is necessary which allows the handling of the microscope via PC.
The selection of sperm under high amplification permits to exclude those spermatozoa with morphological abnormalities and therefore improves significantly the reproductive outcome.
When is IMSI recommended?
The application of IMSI is recommended in cases with bad sperm quality to improve the selection of spermatozoon, which will be used for the fertilisation of the egg.
- Severe Teratozoospermia
- Elevated levels of DNA fragmentation of the sperm